• Western blot analysis of extracts of A-549 cells, using NNMT antibody (STJ28756) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HepG2 cells using NNMT rabbit polyclonal antibody (STJ28756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using NNMT rabbit polyclonal antibody (STJ28756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using NNMT rabbit polyclonal antibody (STJ28756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using NNMT rabbit polyclonal antibody (STJ28756) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NNMT antibody (1-264) (STJ28756)

SKU:
STJ28756

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NNMT (1-264) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NNMT
Gene ID: 4837
Uniprot ID: NNMT_HUMAN
Immunogen Region: 1-264
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human NNMT (NP_006160.1).
Immunogen Sequence: MESGFTSKDTYLSHFNPRDY LEKYYKFGSRHSAESQILKH LLKNLFKIFCLDGVKGDLLI DIGSGPTIYQLLSACESFKE IVVTDYSDQNLQELEKWLKK EPEAFDWSPVVTYVCDLEGN RVKGPEKEEKLRQAVKQVLK CDVTQSQPLGAVPLPPADCV LSTLCLDAACPDLPTYCRAL RNLGSLLKPGGFLVIMDALK SSYYMIGEQKFSSLPLGREA VEAAVKEAGYTIEWFEVIS
Tissue Specificity Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
Post Translational Modifications Deiminated by PADI1 and PADI2.
Function Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway. Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase. Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification.
Protein Name Nicotinamide N-Methyltransferase
Database Links Reactome: R-HSA-156581
Reactome: R-HSA-197264
Reactome: R-HSA-2408508
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nicotinamide N-Methyltransferase antibody
Anti-NNMT antibody

Information sourced from Uniprot.org

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