• Western blot analysis of various lysates, using NMNAT1 Rabbit polyclonal antibody (STJ11105701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NMNAT1 antibody (1-150) (STJ11105701)

SKU:
STJ11105701

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 1 (1-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NMNAT1
Gene ID: 64802
Uniprot ID: NMNA1_HUMAN
Immunogen Region: 1-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human NMNAT1 (NP_073624.2).
Immunogen Sequence: MENSEKTEVVLLACGSFNPI TNMHLRLFELAKDYMNGTGR YTVVKGIISPVGDAYKKKGL IPAYHRVIMAELATKNSKWV EVDTWESLQKEWKETLKVLR HHQEKLEASDCDHQQNSPTL ERPGRKRKWTETQDSSQKKS LEPKTKAVPK
Tissue Specificity Widely expressed with highest levels in skeletal muscle, heart and kidney. Also expressed in the liver pancreas and placenta. Widely expressed throughout the brain.
Function Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form.nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Also acts as a cofactor for glutamate and aspartate ADP-ribosylation by directing PARP1 catalytic activity to glutamate and aspartate residues on histones. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following mechanical or toxic insults.
Protein Name Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 1
Nmn/Namn Adenylyltransferase 1
Nicotinamide-Nucleotide Adenylyltransferase 1
Nmn Adenylyltransferase 1
Nicotinate-Nucleotide Adenylyltransferase 1
Namn Adenylyltransferase 1
Database Links Reactome: R-HSA-196807
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nicotinamide/Nicotinic Acid Mononucleotide Adenylyltransferase 1 antibody
Anti-Nmn/Namn Adenylyltransferase 1 antibody
Anti-Nicotinamide-Nucleotide Adenylyltransferase 1 antibody
Anti-Nmn Adenylyltransferase 1 antibody
Anti-Nicotinate-Nucleotide Adenylyltransferase 1 antibody
Anti-Namn Adenylyltransferase 1 antibody
Anti-NMNAT1 antibody
Anti-NMNAT antibody

Information sourced from Uniprot.org

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