• Immunoprecipitation analysis of 600 Mu g extracts of Mouse kidney using 3 Mu g NLRX1 antibody (STJ116270). Western blot was performed from the immunoprecipitate using NLRX1 (STJ116270) at a dilution of 1:1000.
  • Western blot analysis of lysates from Mouse heart, using NLRX1 Rabbit polyclonal antibody (STJ116270) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NLRX1 antibody (87-975) (STJ116270)

SKU:
STJ116270

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NLRX1 (87-975) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NLRX1
Gene ID: 79671
Uniprot ID: NLRX1_HUMAN
Immunogen Region: 87-975
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 87-975 of human NLRX1 (NP_078894.2).
Immunogen Sequence: AEWFSRLPREERQFGPTFAL DTVHVDPVIRESTPDELLRP PAELALEHQPPQAGLPPLAL SQLFNPDACGRRVQTVVLYG TVGTGKSTLVRKMVLDWCYG RLPAFELLIPFSCEDLSSLG PAPASLCQLVAQRYTPLKEV LPLMAAAGSHLLFVLHGLEH LNLDFRLAGTGLCSDPEEPQ EPAAIIVNLLRKYMLPQASI LVTTRPSAIGRIPSKYVGRY GEICGFSDTNLQKLYFQLR
Tissue Specificity Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji and Jurkat cell lines (at protein level).
Function Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction. Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy-related proteins ATG5 and ATG12. Regulates also MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis. Has no inhibitory function on NF-kappa-B signaling pathway, but enhances NF-kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species. Regulates viral mediated-inflammation and energy metabolism in a sex-dependent manner. In females, prevents uncontrolled inflammation and energy metabolism and thus, may contribute to the sex differences observed in infectious and inflammatory diseases.
Protein Name Nlr Family Member X1
Caterpiller Protein 11.3
Clr11.3
Nucleotide-Binding Oligomerization Domain Protein 5
Nucleotide-Binding Oligomerization Domain Protein 9
Database Links Reactome: R-HSA-168928
Reactome: R-HSA-936440
Reactome: R-HSA-9758274
Cellular Localisation Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Nlr Family Member X1 antibody
Anti-Caterpiller Protein 11.3 antibody
Anti-Clr11.3 antibody
Anti-Nucleotide-Binding Oligomerization Domain Protein 5 antibody
Anti-Nucleotide-Binding Oligomerization Domain Protein 9 antibody
Anti-NLRX1 antibody
Anti-NOD5 antibody
Anti-NOD9 antibody

Information sourced from Uniprot.org

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