![Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89110/357564/STJ11100038_1__86724.1713121690.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89110/357564/STJ11100038_1__86724.1713121690.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
Anti-NLRX1 antibody (87-360) (STJ11100038)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NLRX1 (87-360) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NLRX1 |
| Gene ID | 79671 |
| Uniprot ID | NLRX1_HUMAN |
| Immunogen Region | 87-360 |
| Immunogen Sequence | AEWFSRLPREERQFGPTFAL DTVHVDPVIRESTPDELLRP PAELALEHQPPQAGLPPLAL SQLFNPDACGRRVQTVVLYG TVGTGKSTLVRKMVLDWCYG RLPAFELLIPFSCEDLSSLG PAPASLCQLVAQRYTPLKEV LPLMAAAGSHLLFVLHGLEH LNLDFRLAGTGLCSDPEEPQ EPAAIIVNLLRKYMLPQASI LVTTRPSAIGRIPSKYVGRY GEICGFSDTNLQKLYFQLR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 87-360 of human NLRX1 (NP_001269073.1). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji and Jurkat cell lines (at protein level). |
| Function | Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction. Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy-related proteins ATG5 and ATG12. Also regulates MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis. Has no inhibitory function on NF-kappa-B signaling pathway, but enhances NF-kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species. Regulates viral mediated-inflammation and energy metabolism in a sex-dependent manner. In females, prevents uncontrolled inflammation and energy metabolism and thus, may contribute to the sex differences observed in infectious and inflammatory diseases. |
| Protein Name | Nlr Family Member X1Caterpiller Protein 11.3Clr11.3Nucleotide-Binding Oligomerization Domain Protein 5Nucleotide-Binding Oligomerization Domain Protein 9 |
| Database Links | Reactome: R-HSA-168928Reactome: R-HSA-936440Reactome: R-HSA-9758274 |
| Cellular Localisation | Mitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Nlr Family Member X1 antibodyAnti-Caterpiller Protein 11.3 antibodyAnti-Clr11.3 antibodyAnti-Nucleotide-Binding Oligomerization Domain Protein 5 antibodyAnti-Nucleotide-Binding Oligomerization Domain Protein 9 antibodyAnti-NLRX1 antibodyAnti-NOD5 antibodyAnti-NOD9 antibody |
Information sourced from Uniprot.org