| Host: |
Rabbit |
| Applications: |
WB/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NLRX1 (87-975) is suitable for use in Western Blot and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NLRX1 |
| Gene ID: |
79671 |
| Uniprot ID: |
NLRX1_HUMAN |
| Immunogen Region: |
87-975 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 87-975 of human NLRX1 (NP_078894.2). |
| Immunogen Sequence: |
AEWFSRLPREERQFGPTFAL DTVHVDPVIRESTPDELLRP PAELALEHQPPQAGLPPLAL SQLFNPDACGRRVQTVVLYG TVGTGKSTLVRKMVLDWCYG RLPAFELLIPFSCEDLSSLG PAPASLCQLVAQRYTPLKEV LPLMAAAGSHLLFVLHGLEH LNLDFRLAGTGLCSDPEEPQ EPAAIIVNLLRKYMLPQASI LVTTRPSAIGRIPSKYVGRY GEICGFSDTNLQKLYFQLR |
| Tissue Specificity | Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji and Jurkat cell lines (at protein level). |
| Function | Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction. Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy-related proteins ATG5 and ATG12. Regulates also MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis. Has no inhibitory function on NF-kappa-B signaling pathway, but enhances NF-kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species. Regulates viral mediated-inflammation and energy metabolism in a sex-dependent manner. In females, prevents uncontrolled inflammation and energy metabolism and thus, may contribute to the sex differences observed in infectious and inflammatory diseases. |
| Protein Name | Nlr Family Member X1Caterpiller Protein 11.3Clr11.3Nucleotide-Binding Oligomerization Domain Protein 5Nucleotide-Binding Oligomerization Domain Protein 9 |
| Database Links | Reactome: R-HSA-168928Reactome: R-HSA-936440Reactome: R-HSA-9758274 |
| Cellular Localisation | Mitochondrion Outer Membrane |
| Alternative Antibody Names | Anti-Nlr Family Member X1 antibodyAnti-Caterpiller Protein 11.3 antibodyAnti-Clr11.3 antibodyAnti-Nucleotide-Binding Oligomerization Domain Protein 5 antibodyAnti-Nucleotide-Binding Oligomerization Domain Protein 9 antibodyAnti-NLRX1 antibodyAnti-NOD5 antibodyAnti-NOD9 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89110/357564/STJ11100038_1__86724.1713121690.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NLRX1 knockout (KO) HeLa cells, using [KO Validated] NLRX1 Rabbit polyclonal antibody (STJ11100038) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
