• Western blot analysis of extracts of various cell lines, using NLRP12 antibody (STJ117830) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NLRP12 antibody (862-1061) (STJ117830)

SKU:
STJ117830

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NLRP12 (862-1061) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NLRP12
Gene ID: 91662
Uniprot ID: NAL12_HUMAN
Immunogen Region: 862-1061
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 862-1061 of human NLRP12 (NP_653288.1).
Immunogen Sequence: WLKICRLTAAACDELASTLS VNQSLRELDLSLNELGDLGV LLLCEGLRHPTCKLQTLRLG ICRLGSAACEGLSVVLQANH NLRELDLSFNDLGDWGLWLL AEGLQHPACRLQKLWLDSCG LTAKACENLYFTLGINQTLT DLYLTNNALGDTGVRLLCKR LSHPGCKLRVLWLFGMDLNK MTHSRLAALRVTKPYLDIGC
Tissue Specificity Detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes.
Function Plays an essential role as an potent mitigator of inflammation. Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways. Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway. In turn, promotes bacterial tolerance. Inhibits also the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation. Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue.
Protein Name Nacht - Lrr And Pyd Domains-Containing Protein 12
Monarch-1
Pyrin-Containing Apaf1-Like Protein 7
Regulated By Nitric Oxide
Database Links Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nacht - Lrr And Pyd Domains-Containing Protein 12 antibody
Anti-Monarch-1 antibody
Anti-Pyrin-Containing Apaf1-Like Protein 7 antibody
Anti-Regulated By Nitric Oxide antibody
Anti-NLRP12 antibody
Anti-NALP12 antibody
Anti-PYPAF7 antibody
Anti-RNO antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance