| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NLRC4 (85-170) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NLRC4 |
| Gene ID: |
58484 |
| Uniprot ID: |
NLRC4_HUMAN |
| Immunogen Region: |
85-170 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 85-170 of human NLRC4 (NP_001186067.1). |
| Immunogen Sequence: |
NGQSLFHQTSEGDLDDLAQD LKDLYHTPSFLNFYPLGEDI DIIFNLKSTFTEPVLWRKDQ HHHRVEQLTLNGLLQALQSP CIIEGE |
| Tissue Specificity | Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain. |
| Post Translational Modifications | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro. |
| Function | Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. |
| Protein Name | Nlr Family Card Domain-Containing Protein 4Card - Lrr - And Nacht-Containing ProteinCed-4-Like Protein ClanCaspase Recruitment Domain-Containing Protein 12Ice Protease-Activating FactorIpaf |
| Database Links | Reactome: R-HSA-6803207Reactome: R-HSA-844623 |
| Cellular Localisation | CytoplasmCytosolInflammasome |
| Alternative Antibody Names | Anti-Nlr Family Card Domain-Containing Protein 4 antibodyAnti-Card - Lrr - And Nacht-Containing Protein antibodyAnti-Ced-4-Like Protein Clan antibodyAnti-Caspase Recruitment Domain-Containing Protein 12 antibodyAnti-Ice Protease-Activating Factor antibodyAnti-Ipaf antibodyAnti-NLRC4 antibodyAnti-CARD12 antibodyAnti-CLAN antibodyAnti-CLAN1 antibodyAnti-IPAF antibodyAnti-UNQ6189 antibodyAnti-PRO20215 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
