• Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-NISCH antibody (234-284 aa) (STJ196047)

SKU:
STJ196047

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nischarin (234-284 aa) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NISCH
Gene ID: 11188
Uniprot ID: NISCH_HUMAN
Immunogen Region: 234-284 aa
Specificity: This antibody detects endogenous levels of NISCH at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human NISCH at the amino acid range 234-284
Function Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.
Protein Name Nischarin
Imidazoline Receptor 1
I-1
Ir1
Imidazoline Receptor Antisera-Selected Protein
Hiras
Imidazoline-1 Receptor
I1r
Imidazoline-1 Receptor Candidate Protein
I-1 Receptor Candidate Protein
I1r Candidate Protein
Database Links Reactome: R-HSA-9013149
Reactome: R-HSA-9696264
Reactome: R-HSA-9696270
Cellular Localisation Cell Membrane
Cytoplasm
Early Endosome
Recycling Endosome
Enriched In The Early/Sorting And Recycling Endosomes
Colocalized In Early/Sorting Endosomes With Eea1 And Snx2 And In Recycling Endosomes With Transferrin Receptor
Detected In The Perinuclear Region Partially Associated With Punctate Structures
Colocalizes With Pak1 In Cytoplasm
Vesicular Structures In The Perinuclear Area And Membrane Ruffles
Colocalizes With Rac1 In The Cytoplasm And Vesicles Structures
Colocalized With Mapk1 And Mapk3 In Rvlm Neurons
Alternative Antibody Names Anti-Nischarin antibody
Anti-Imidazoline Receptor 1 antibody
Anti-I-1 antibody
Anti-Ir1 antibody
Anti-Imidazoline Receptor Antisera-Selected Protein antibody
Anti-Hiras antibody
Anti-Imidazoline-1 Receptor antibody
Anti-I1r antibody
Anti-Imidazoline-1 Receptor Candidate Protein antibody
Anti-I-1 Receptor Candidate Protein antibody
Anti-I1r Candidate Protein antibody
Anti-NISCH antibody
Anti-IRAS antibody
Anti-KIAA0975 antibody

Information sourced from Uniprot.org

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