• STJ72250 (2µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ72250 Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
  • STJ72250 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

Anti-NIPBL antibody (889-902) (STJ72250)

SKU:
STJ72250-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IHC/IF
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-NIPBL (889-902) is suitable for use in ELISA, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2µg/ml
IF-Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NIPBL
Gene ID: 25836
Uniprot ID: NIPBL_HUMAN
Immunogen Region: 889-902
Accession Number: NP_597677.2; NP_056199.2
Specificity: This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).
Immunogen Sequence: RPDSPRVKQGDSNK
Function Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin. Plays a role in cohesin loading at sites of DNA damage. Its recruitment to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitment to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner. Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others.
Protein Name Nipped-B-Like Protein
Delangin
Scc2 Homolog
Database Links Reactome: R-HSA-2470946
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Nipped-B-Like Protein antibody
Anti-Delangin antibody
Anti-Scc2 Homolog antibody
Anti-NIPBL antibody
Anti-IDN3 antibody
Anti-SCC2 antibody

Information sourced from Uniprot.org

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