| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NINL (1-190) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NINL |
| Gene ID: |
22981 |
| Uniprot ID: |
NINL_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NINL (NP_079452.3). |
| Immunogen Sequence: |
MDEEENHYVSQLREVYSSCD TTGTGFLDRQELTQLCLKLH LEQQLPVLLQTLLGNDHFAR VNFEEFKEGFVAVLSSNAGV RPSDEDSSSLESAASSAIPP KYVNGSKWYGRRSRPELCDA ATEARRVPEQQTQASLKSHL WRSASLESVESPKSDEEAES TKEAQNELFEAQGQLQTWDS EDFGSPQKSC |
| Tissue Specificity | Expressed in KYSE-150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina. |
| Post Translational Modifications | Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin. Ubiquitinated by the APC/C complex leading to its degradation. |
| Function | Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery.it also interferes with mitotic spindle assembly. Involved in vesicle transport in photoreceptor cells. May play a role in ovarian carcinogenesis. |
| Protein Name | Ninein-Like Protein |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeIn Interphase CellsNinl Is Transported To The Centrosomes By The Dynein-Dynactin Motor ComplexDuring Centrosome MaturationPlk1 Directly Phosphorylates Ninl Resulting In Its Release Into The Cytoplasm |
| Alternative Antibody Names | Anti-Ninein-Like Protein antibodyAnti-NINL antibodyAnti-KIAA0980 antibodyAnti-NLP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")