• Western blot analysis of various lysates using NIFK Rabbit polyclonal antibody (STJ118044) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-NIFK antibody (180-260) (STJ118044)

SKU:
STJ118044

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NIFK (180-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NIFK
Gene ID: 84365
Uniprot ID: MK67I_HUMAN
Immunogen Region: 180-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human NIFK (NP_115766.3).
Immunogen Sequence: GIDYDFPSLILQKTESISKT NRQTSTKGQVLRKKKKKVSG TLDTPEKTVDSQGPTPVCTP TFLERRKSQVAELNDDDKDD E
Post Translational Modifications Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
Protein Name Mki67 Fha Domain-Interacting Nucleolar Phosphoprotein
Nucleolar Phosphoprotein Nopp34
Nucleolar Protein Interacting With The Fha Domain Of Pki-67
Hnifk
Cellular Localisation Nucleus
Nucleolus
Chromosome
Localizes To Mitotic Chromosomes In Conjunction With Mki67
Alternative Antibody Names Anti-Mki67 Fha Domain-Interacting Nucleolar Phosphoprotein antibody
Anti-Nucleolar Phosphoprotein Nopp34 antibody
Anti-Nucleolar Protein Interacting With The Fha Domain Of Pki-67 antibody
Anti-Hnifk antibody
Anti-NIFK antibody
Anti-MKI67IP antibody
Anti-NOPP34 antibody

Information sourced from Uniprot.org

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