Anti-Nicotinic Acetylcholine Receptor alpha 7 antibody (7-2) {Biotin} (STJ501865)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationBiotin
IsotypeIgG
ImmunogenSynthetic peptide corresponding to exon 5 of alpha 7-2 nAChRs protein.
General Information
| Short Description | Rabbit polyclonal antibody anti-Nicotinic Acetylcholine Receptor alpha 7 (7-2) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Applications | ELISA/IP/WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Biotin |
| Concentration | 0.75-0.95 ยตg/ยตl |
| Purification | Affinity Purified |
| Dilution Range | WB: 1:250DB: 1:10, 000ELISA: 1:10, 000IP: 1:200IHC: 1:50-1:150 |
| Formulation | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Storage Instruction | Store at-20ยฐC for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Chrna7 |
| Gene ID | 25302 |
| Uniprot ID | ACHA7_RAT |
| Immunogen | Synthetic peptide corresponding to exon 5 of alpha 7-2 nAChRs protein. |
| Immunogen Region | 7-2 |
Additional Info
| Post Translational Modifications | Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane. |
| Function | After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Alpha-7 |
| Database Links | Reactome: R-RNO-629594 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell MembraneRic3 Promotes Its Trafficking To The Cell MembraneTmem35a/Nacho Promotes Its Trafficking To The Cell Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Alpha-7 antibodyAnti-Chrna7 antibodyAnti-Acra7 antibody |
Information sourced from Uniprot.org