Anti-Nicastrin, N-terminal antibody (N-Term) (STJA0040965)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide (RGNSVERKIYIPL-C) corresponding to human Nicastrin [32-44] in the N-terminal region conjugated via additional C-terminal Cys to Diphtheria toxoid.
General Information
| Short Description | Rabbit polyclonal anti-Nicastrin, N-terminal (N-Term) for use in WB and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 3-10 ยตg/mL |
| Formulation | Lyophilized from PBS, pH 7.4. Contains no preservative. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 500 ยตL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. Short term storage at 2-8ยฐC for one week. At-20ยฐC as an undiluted liquid for up to 12 months. |
Target Information
| Gene Symbol | NCSTN |
| Gene ID | 23385 |
| Uniprot ID | NICA_HUMAN |
| Immunogen | A synthetic peptide (RGNSVERKIYIPL-C) corresponding to human Nicastrin [32-44] in the N-terminal region conjugated via additional C-terminal Cys to Diphtheria toxoid. |
| Immunogen Region | N-Term |
| Immunogen Sequence | Human |
| Specificity | Confirmed by WB using peptide absorption. |
Additional Info
| Tissue Specificity | Detected in brain (at protein level). Widely expressed. |
| Post Translational Modifications | N-glycosylated. |
| Function | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. |
| Protein Name | Nicastrin |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-1474228Reactome: R-HSA-193692Reactome: R-HSA-205043Reactome: R-HSA-2122948Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-2979096Reactome: R-HSA-3928665Reactome: R-HSA-6798695Reactome: R-HSA-9013507Reactome: R-HSA-9013700Reactome: R-HSA-9017802Reactome: R-HSA-977225Reactome: R-HSA-9839383 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCytoplasmic Vesicle MembraneMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Nicastrin antibodyAnti-NCSTN antibodyAnti-KIAA0253 antibodyAnti-UNQ1874 antibodyAnti-PRO4317 antibody |
Information sourced from Uniprot.org