• Western blot analysis of 293F-NHLH1, using NHLH1 rabbit polyclonal antibody (STJ118141) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 300ug extracts of 293F-NHLH1-Avi-His (C) cells using 3ug NHLH1 rabbit polyclonal antibody (STJ118141 1:300). Western blot was performed from the immunoprecipitate using NHLH1 rabbit polyclonal antibody antibody (STJ118141) at a dilition of 1:3000.

Anti-NHLH1 antibody (1-60) (STJ118141)

SKU:
STJ118141

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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NHLH1 (1-60) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IP 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NHLH1
Gene ID: 4807
Uniprot ID: HEN1_HUMAN
Immunogen Region: 1-60
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human NHLH1 (NP_005589.1).
Immunogen Sequence: MMLNSDTMELDLPPTHSETE SGFSDCGGGAGPDGAGPGGP GGGQARGPEPGEPGRKDLQH
Function May serve as DNA-binding protein and may be involved in the control of cell-type determination, possibly within the developing nervous system.
Protein Name Helix-Loop-Helix Protein 1
Hen-1
Class A Basic Helix-Loop-Helix Protein 35
Bhlha35
Nescient Helix Loop Helix 1
Nscl-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Helix-Loop-Helix Protein 1 antibody
Anti-Hen-1 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 35 antibody
Anti-Bhlha35 antibody
Anti-Nescient Helix Loop Helix 1 antibody
Anti-Nscl-1 antibody
Anti-NHLH1 antibody
Anti-BHLHA35 antibody
Anti-HEN1 antibody

Information sourced from Uniprot.org

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