Anti-NHERF2-Isoform b/e antibody (Internal) (STJ70794)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-NHERF2-Isoform b/e (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in lysates of cell lines HEK293 and Daudi (calculated MW of 36.2kDa according to NP_004776.3). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | NHERF2 |
| Gene ID | 9351 |
| Uniprot ID | NHRF2_HUMAN |
| Accession Number | NP_004776.3; NP_001239005.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | DLPGSDKDTEESGLH |
| Specificity | This antibody is expected to recognize isoforms b and e (NP_004776.3 and NP_001239005.1) only. |
Additional Info
| Function | Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May also act as scaffold protein in the nucleus. |
| Protein Name | Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf2Nherf-2Nhe3 Kinase A Regulatory Protein E3karpSry-Interacting Protein 1Sip-1Sodium-Hydrogen Exchanger Regulatory Factor 2Solute Carrier Family 9 Isoform A3 Regulatory Factor 2Tyrosine Kinase Activator Protein 1Tka-1 |
| Cellular Localisation | Endomembrane SystemPeripheral Membrane ProteinNucleusApical Cell MembraneLocalizes With Ezr And Podxl At The Apical Cell Membrane Of Glomerular Epithelium Cells And The Sides Of The Food ProcessesNuclearIn A Punctate Pattern |
| Alternative Antibody Names | Anti-Na(+/H(+ Exchange Regulatory Cofactor Nhe-Rf2 antibodyAnti-Nherf-2 antibodyAnti-Nhe3 Kinase A Regulatory Protein E3karp antibodyAnti-Sry-Interacting Protein 1 antibodyAnti-Sip-1 antibodyAnti-Sodium-Hydrogen Exchanger Regulatory Factor 2 antibodyAnti-Solute Carrier Family 9 Isoform A3 Regulatory Factor 2 antibodyAnti-Tyrosine Kinase Activator Protein 1 antibodyAnti-Tka-1 antibodyAnti-NHERF2 antibodyAnti-SLC9A3R2 antibody |
Information sourced from Uniprot.org