| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NGLY1 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NGLY1 |
| Gene ID: |
55768 |
| Uniprot ID: |
NGLY1_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2). |
| Immunogen Sequence: |
MAAAALGSSSGSASPAVAEL CQNTPETFLEASKLLLTYAD NILRNPNDEKYRSIRIGNTA FSTRLLPVRGAVECLFEMGF EEGETHLIFPKKASVEQLQK IRDLIAIERSSRLDGSNKSH KVKSSQQPAASTQLPTTPSS NPSGLNQHTRNRQGQSSDPP SASTVAADSAILEVLQSNIQ HVLVYENPALQEKALACIPV QELKRKSQEKLSRARKLDKG INISDEDFLLLELLHWFKE |
| Function | Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. |
| Protein Name | Peptide-N(4-(N-Acetyl-Beta-Glucosaminylasparagine AmidasePngaseHpngaseN-Glycanase 1Peptide -N-Glycanase |
| Database Links | Reactome: R-HSA-532668 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Peptide-N(4-(N-Acetyl-Beta-Glucosaminylasparagine Amidase antibodyAnti-Pngase antibodyAnti-Hpngase antibodyAnti-N-Glycanase 1 antibodyAnti-Peptide -N-Glycanase antibodyAnti-NGLY1 antibodyAnti-PNG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
