Anti-NGLY1 antibody (1-300 aa) [SRM5382] (STJ11107293)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal NGLY1 (1-300 aa) antibody for use in IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5382 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | IHC-P:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NGLY1 |
| Gene ID | 55768 |
| Uniprot ID | NGLY1_HUMAN |
| Immunogen Region | 1-300 aa |
| Immunogen Sequence | MAAAALGSSSGSASPAVAEL CQNTPETFLEASKLLLTYAD NILRNPNDEKYRSIRIGNTA FSTRLLPVRGAVECLFEMGF EEGETHLIFPKKASVEQLQK IRDLIAIERSSRLDGSNKSH KVKSSQQPAASTQLPTTPSS NPSGLNQHTRNRQGQSSDPP SASTVAADSAILEVLQSNIQ HVLVYENPALQEKALACIPV QELKRKSQEKLSRARKLDKG INISDEDFLLLELLHWFKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NGLY1 (NP_060767.2). |
Additional Info
| Function | Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. |
| Protein Name | Peptide-N(4-(N-Acetyl-Beta-Glucosaminylasparagine AmidasePngaseHpngaseN-Glycanase 1Peptide -N-Glycanase |
| Database Links | Reactome: R-HSA-532668 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Peptide-N(4-(N-Acetyl-Beta-Glucosaminylasparagine Amidase antibodyAnti-Pngase antibodyAnti-Hpngase antibodyAnti-N-Glycanase 1 antibodyAnti-Peptide -N-Glycanase antibodyAnti-NGLY1 antibodyAnti-PNG1 antibody |
Information sourced from Uniprot.org