Anti-NG2 antibody (STJ13100197)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from rat NG2 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-NG2 for use in IHC and WB in Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Cspg4 |
| Gene ID | 81651 |
| Uniprot ID | CSPG4_RAT |
| Immunogen | A synthetic peptide from rat NG2 conjugated to blue carrier protein was used as the antigen. |
| Specificity | Specific for NG2. |
Additional Info
| Post Translational Modifications | N-glycosylated. O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. |
| Function | Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. |
| Protein Name | Chondroitin Sulfate Proteoglycan 4Chondroitin Sulfate Proteoglycan Ng2Hsn Tumor-Specific Antigen |
| Database Links | Reactome: R-RNO-1971475Reactome: -RNO-2022870Reactome: -RNO-2022923Reactome: -RNO-2024101 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExtracellular SideApical Cell MembraneCell ProjectionLamellipodium MembraneCell SurfaceLocalizes To The Plasma Membrane Of OligodendrocytesLocalized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By PrkcaLocalizes To The Retraction FibersA Fraction May Undergo Cell Surface Proteolysis And Secretion |
| Alternative Antibody Names | Anti-Chondroitin Sulfate Proteoglycan 4 antibodyAnti-Chondroitin Sulfate Proteoglycan Ng2 antibodyAnti-Hsn Tumor-Specific Antigen antibodyAnti-Cspg4 antibodyAnti-Ng2 antibody |
Information sourced from Uniprot.org