• Western blot analysis of extracts of various cell lines, using NFYB antibody (STJ29434) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NFYB antibody (1-207) (STJ29434)

SKU:
STJ29434

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFYB (1-207) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFYB
Gene ID: 4801
Uniprot ID: NFYB_HUMAN
Immunogen Region: 1-207
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1).
Immunogen Sequence: MTMDGDSSTTDASQLGISAD YIGGSHYVIQPHDDTEDSMN DHEDTNGSKESFREQDIYLP IANVARIMKNAIPQTGKIAK DAKECVQECVSEFISFITSE ASERCHQEKRKTINGEDILF AMSTLGFDSYVEPLKLYLQK FREAMKGEKGIGGAVTATDG LSEELTEEAFTNQLPAGLIT TDGQQQNVMVYTTSYQQISG VQQIQFS
Post Translational Modifications Monoubiquitination at Lys-140 plays an important role in transcriptional activation by allowing the deposition of histone H3 methylations as well as histone H2B monoubiquitination at 'Lys-121'.
Function Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
Protein Name Nuclear Transcription Factor Y Subunit Beta
Caat Box Dna-Binding Protein Subunit B
Nuclear Transcription Factor Y Subunit B
Nf-Yb
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2426168
Reactome: R-HSA-380994
Reactome: R-HSA-381183
Reactome: R-HSA-9614657
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Transcription Factor Y Subunit Beta antibody
Anti-Caat Box Dna-Binding Protein Subunit B antibody
Anti-Nuclear Transcription Factor Y Subunit B antibody
Anti-Nf-Yb antibody
Anti-NFYB antibody
Anti-HAP3 antibody

Information sourced from Uniprot.org

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