• Western blot analysis of extracts of various cell lines, using NFU1 antibody (STJ29177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NFU1 antibody (1-230) (STJ29177)

SKU:
STJ29177

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFU1 (1-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFU1
Gene ID: 27247
Uniprot ID: NFU1_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human NFU1 (NP_001002755.1).
Immunogen Sequence: MAATARRGWGAAAVAAGLRR RFCHMLKNPYTIKKQPLHQF VQRPLFPLPAAFYHPVRYMF IQTQDTPNPNSLKFIPGKPV LETRTMDFPTPAAAFRSPLA RQLFRIEGVKSVFFGPDFIT VTKENEELDWNLLKPDIYAT IMDFFASGLPLVTEETPSGE AGSEEDDEVVAMIKELLDTR IRPTVQEDGGDVIYKGFEDG IVQLKLQGSCTSCPSSIITL KNGIQNMLQF
Tissue Specificity Ubiquitous. Expression in adult lung is weak compared to fetal lung.
Function Iron-sulfur cluster scaffold protein which can assemble 4Fe-4S clusters and deliver them to target proteins.
Protein Name Nfu1 Iron-Sulfur Cluster Scaffold Homolog - Mitochondrial
Hira-Interacting Protein 5
Cellular Localisation Mitochondrion
Cytoplasm
Cytosol
Alternative Antibody Names Anti-Nfu1 Iron-Sulfur Cluster Scaffold Homolog - Mitochondrial antibody
Anti-Hira-Interacting Protein 5 antibody
Anti-NFU1 antibody
Anti-HIRIP5 antibody
Anti-CGI-33 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance