• Western blot analysis of extracts of various cell lines, using NFKBIE antibody (STJ110741) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NFKBIE antibody (140-280) (STJ110741)

SKU:
STJ110741

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFKBIE (140-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIE
Gene ID: 4794
Uniprot ID: IKBE_HUMAN
Immunogen Region: 140-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-280 of human NFKBIE (NP_004547.2).
Immunogen Sequence: MSEARKGPDEAEESQYDSGI ESLRSLRSLPESTSAPASGP SDGSPQPCTHPPGPVKEPQE KEDADGERADSTYGSSSLTY TLSLLGGPEAEDPAPRLPLP HVGALSPQQLEALTYISEDG DTLVHLAVIHEAPAVLLCCL A
Tissue Specificity Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Post Translational Modifications Serine phosphorylated.followed by proteasome-dependent degradation.
Function Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.
Protein Name Nf-Kappa-B Inhibitor Epsilon
Nf-Kappa-Bie
I-Kappa-B-Epsilon
Ikb-E
Ikb-Epsilon
Ikappabepsilon
Database Links Reactome: R-HSA-1169091
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Epsilon antibody
Anti-Nf-Kappa-Bie antibody
Anti-I-Kappa-B-Epsilon antibody
Anti-Ikb-E antibody
Anti-Ikb-Epsilon antibody
Anti-Ikappabepsilon antibody
Anti-NFKBIE antibody
Anti-IKBE antibody

Information sourced from Uniprot.org

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