• Immunohistochemistry analysis of paraffin-embedded human kidney cancer using NFKBIB antibody (STJ28344) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using NFKBIB antibody (STJ28344) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using NFKBIB antibody (STJ28344) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NFKBIB antibody (STJ28344) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-NFKBIB antibody (1-356) (STJ28344)

SKU:
STJ28344

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFKBIB (1-356) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIB
Gene ID: 4793
Uniprot ID: IKBB_HUMAN
Immunogen Region: 1-356
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-356 of human NFKBIB (NP_002494.2).
Immunogen Sequence: MAGVACLGKAADADEWCDSG LGSLGPDAAAPGGPGLGAEL GPGLSWAPLVFGYVTEDGDT ALHLAVIHQHEPFLDFLLGF SAGTEYMDLQNDLGQTALHL AAILGETSTVEKLYAAGAGL CVAERRGHTALHLACRVGAH ACARALLQPRPRRPREAPDT YLAQGPDRTPDTNHTPVALY PDSDLEKEEEESEEDWKLQL EAENYEGHTPLHVAVIHKDV EMVRLLRDAGADLDKPEPT
Tissue Specificity Expressed in all tissues examined.
Post Translational Modifications Phosphorylated by RPS6KA1.followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
Function Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Protein Name Nf-Kappa-B Inhibitor Beta
Nf-Kappa-Bib
I-Kappa-B-Beta
Ikb-B
Ikb-Beta
Ikappabbeta
Thyroid Receptor-Interacting Protein 9
Tr-Interacting Protein 9
Trip-9
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-445989
Reactome: R-HSA-933542
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Beta antibody
Anti-Nf-Kappa-Bib antibody
Anti-I-Kappa-B-Beta antibody
Anti-Ikb-B antibody
Anti-Ikb-Beta antibody
Anti-Ikappabbeta antibody
Anti-Thyroid Receptor-Interacting Protein 9 antibody
Anti-Tr-Interacting Protein 9 antibody
Anti-Trip-9 antibody
Anti-NFKBIB antibody
Anti-IKBB antibody
Anti-TRIP9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance