• Western blot analysis of extracts from normal (control) and I Kappa B Alpha knockout (KO) 293T cells, using I Kappa B Alpha antibody (STJ113118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of various cell lines, using [KO Validated] I Kappa B Alpha antibody (STJ113118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat liver, using [KO Validated] I Kappa B Alpha antibody (STJ113118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug I Kappa B Alpha antibody (STJ113118). Western blot was performed from the immunoprecipitate using I Kappa B Alpha antibody (STJ113118) at a dilition of 1:500.

Anti-NFKBIA antibody (201-317) (STJ113118)

SKU:
STJ113118

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-I Kappa B Alpha (201-317) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIA
Gene ID: 4792
Uniprot ID: IKBA_HUMAN
Immunogen Region: 201-317
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 201-317 of human I Kappa B Alpha (NP_065390.1).
Immunogen Sequence: LLVSLGADVNAQEPCNGRTA LHLAVDLQNPDLVSLLLKCG ADVNRVTYQGYSPYQLTWGR PSTRIQQQLGQLTLENLQML PESEDEESYDTESEFTEFTE
Post Translational Modifications Phosphorylated at Ser-32 and Ser-36 by IKKA/CHUK and IKKB/IKBKB.disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by the SCF(FBXW11) and SCF(BTRC) complexes, leading to polyubiquitination and subsequent degradation. Phosphorylated at Ser-32 in response to FK506 treatment: phosphorylation is independent of IKKA/CHUK and IKKB/IKBKB and promotes NFKBIA degradation, followed by NF-kappa-B activation. Phosphorylated at Tyr-42: its effect is however unclear. According to a report, phosphorylation at Tyr-42 activates NF-kappa-B without triggering proteolytic degradation of NFKBIA. According to another publication, phosphorylation at Tyr-42 inhibits NF-kappa-B activity by preventing phosphorylation at Ser-32 and Ser-36 and subsequent ubiquitination and degradation. Polyubiquitinated at Lys-21 and/or Lys-22 following phosphorylation at Ser-32 and Ser-36. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by the SCF(BTRC) complex following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by USP38, leading to NF-kappa-B inhibition. Sumoylated.sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. Hydroxylated by HIF1AN. (Microbial infection) Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Protein Name Nf-Kappa-B Inhibitor Alpha
I-Kappa-B-Alpha
Ikb-Alpha
Ikappabalpha
Major Histocompatibility Complex Enhancer-Binding Protein Mad3
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-202424
Reactome: R-HSA-209560
Reactome: R-HSA-2871837
Reactome: R-HSA-445989
Reactome: R-HSA-4755510
Reactome: R-HSA-5603029
Reactome: R-HSA-5607764
Reactome: R-HSA-5689880
Reactome: R-HSA-9020702
Reactome: R-HSA-933542
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Nucleus And The Cytoplasm By A Nuclear Localization Signal (Nls) And A Crm1-Dependent Nuclear Export
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Alpha antibody
Anti-I-Kappa-B-Alpha antibody
Anti-Ikb-Alpha antibody
Anti-Ikappabalpha antibody
Anti-Major Histocompatibility Complex Enhancer-Binding Protein Mad3 antibody
Anti-NFKBIA antibody
Anti-IKBA antibody
Anti-MAD3 antibody
Anti-NFKBI antibody

Information sourced from Uniprot.org

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