• Western blot analysis of extracts of mouse brain, using NFIA antibody (STJ24756) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

Anti-NFIA antibody (STJ24756)

SKU:
STJ24756
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Host: Rabbit
Applications: WB
Reactivity: Human, Mouse
Short Description: Rabbit polyclonal primary antibody against NFIA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:200-1:2000
Uniprot ID: NFIA_HUMAN
Gene ID: 4774
Gene Symbol: NFIA
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-330 of human NFIA (NP_005586.1).
Background This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene.
Function Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Database Links Reactome: R-HSA-73980
Cellular Localisation Nucleus
Protein Name Nuclear Factor 1 A-Type
Nf1-A
Nuclear Factor 1/A
Ccaat-Box-Binding Transcription Factor
Ctf
Nuclear Factor I/A
Nf-I/A
Nfi-A
Tggca-Binding Protein
Alternative Names Anti-Nuclear Factor 1 A-Type antibody
Anti-Nf1-A antibody
Anti-Nuclear Factor 1/A antibody
Anti-Ccaat-Box-Binding Transcription Factor antibody
Anti-Ctf antibody
Anti-Nuclear Factor I/A antibody
Anti-Nf-I/A antibody
Anti-Nfi-A antibody
Anti-Tggca-Binding Protein antibody
Anti-NFIA antibody
Anti-KIAA1439 antibody

Information sourced from uniprot.org

6 months standard