• Immunohistochemistry analysis of NFE2L3 in paraffin-embedded human placenta tissue using NFE2L3 Rabbit polyclonal antibody (STJ118206) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of NFE2L3 in paraffin-embedded human colon carcinoma tissue using NFE2L3 Rabbit polyclonal antibody (STJ118206) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of NFE2L3 in paraffin-embedded rat colon tissue using NFE2L3 Rabbit polyclonal antibody (STJ118206) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using NFE2L3 Rabbit polyclonal antibody (STJ118206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFE2L3 antibody (330-694) (STJ118206)

SKU:
STJ118206

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFE2L3 (330-694) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFE2L3
Gene ID: 9603
Uniprot ID: NF2L3_HUMAN
Immunogen Region: 330-694
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 330-694 of human NFE2L3 (NP_004280.5).
Immunogen Sequence: DPTARTSQSQEPFLQLNSHT TNPEQTLPGTNLTGFLSPVD NHMRNLTSQDLLYDLDINIF DEINLMSLATEDNFDPIDVS QLFDEPDSDSGLSLDSSHNN TSVIKSNSSHSVCDEGAIGY CTDHESSSHHDLEGAVGGYY PEPSKLCHLDQSDSDFHGDL TFQHVFHNHTYHLQPTAPES TSEPFPWPGKSQKIRSRYLE DTDRNLSRDEQRAKALHIPF S
Tissue Specificity Highly expressed in human placenta and also in B-cell and monocyte cell lines. Low expression in heart, brain, lung, skeletal muscle, kidney and pancreas.
Function Activates erythroid-specific, globin gene expression.
Protein Name Nuclear Factor Erythroid 2-Related Factor 3
Nf-E2-Related Factor 3
Nfe2-Related Factor 3
Nuclear Factor - Erythroid Derived 2 - Like 3
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Nuclear Factor Erythroid 2-Related Factor 3 antibody
Anti-Nf-E2-Related Factor 3 antibody
Anti-Nfe2-Related Factor 3 antibody
Anti-Nuclear Factor - Erythroid Derived 2 - Like 3 antibody
Anti-NFE2L3 antibody
Anti-NRF3 antibody

Information sourced from Uniprot.org

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