| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NFE2L1 (515-772) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFE2L1 |
| Gene ID: |
4779 |
| Uniprot ID: |
NF2L1_HUMAN |
| Immunogen Region: |
515-772 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 515-772 of human NFE2L1 (NP_003195.1). |
| Immunogen Sequence: |
SSSFSEEGAVGYSSDSETLD LEEAEGAVGYQPEYSKFCRM SYQDPAQLSCLPYLEHVGHN HTYNMAPSALDSADLPPPSA LKKGSKEKQADFLDKQMSRD EHRARAMKIPFTNDKIINLP VEEFNELLSKYQLSEAQLSL IRDIRRRGKNKMAAQNCRKR KLDTILNLERDVEDLQRDKA RLLREKVEFLRSLRQMKQKV QSLYQEVFGRLRDENGRPYS PSQYALQYAGDGSVLLIPR |
| Post Translational Modifications | Endoplasmic reticulum membrane sensor NFE2L1: Cleaved at Leu-104 by the aspartyl protease DDI2 following retrotranslocation, releasing the protein from the endoplasmic reticulum membrane and forming the transcription factor NRF1 that translocates into the nucleus. Ubiquitination is prerequisite for cleavage by aspartyl protease DDI2. Endoplasmic reticulum membrane sensor NFE2L1: N-glycosylated in normal conditions, when it has a single-pass type II membrane protein topology, with the DNA-binding domain facing the endoplasmic reticulum lumen. Deglycosylated during retrotranslocation to the cytosolic side of the membrane, to have a single-pass type III membrane protein topology with the major part of the protein facing the cytosol. Endoplasmic reticulum membrane sensor NFE2L1: Ubiquitinated by the SCF(FBXW7) complex and SYVN1/HRD1, leading to its degradation by the proteasome. Ubiquitinated during retrotranslocation to the cytosolic side of the membrane: ubiquitination does not lead to degradation and is required for processing by the aspartyl protease DDI2 and subsequent release from the endoplasmic reticulum membrane. Transcription factor NRF1: Phosphorylation by CK2 at Ser-528 inhibits transcription factor activity, possibly by affecting DNA-binding activity. Phosphorylation at Ser-599 is required for interaction with CEBPB. Transcription factor NRF1: Ubiquitinated by the SCF(BTRC) complex in the nucleus, leading to its degradation by the proteasome. |
| Function | Endoplasmic reticulum membrane sensor NFE2L1: Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor. Constitutes a precursor of the transcription factor NRF1. Able to detect various cellular stresses, such as cholesterol excess, oxidative stress or proteasome inhibition. In response to stress, it is released from the endoplasmic reticulum membrane following cleavage by the protease DDI2 and translocates into the nucleus to form the transcription factor NRF1. Acts as a key sensor of cholesterol excess: in excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal, such as CD36. Involved in proteasome homeostasis: in response to proteasome inhibition, it is released from the endoplasmic reticulum membrane, translocates to the nucleus and activates expression of genes encoding proteasome subunits. Transcription factor NRF1: CNC-type bZIP family transcription factor that translocates to the nucleus and regulates expression of target genes in response to various stresses. Heterodimerizes with small-Maf proteins (MAFF, MAFG or MAFK) and binds DNA motifs including the antioxidant response elements (AREs), which regulate expression of genes involved in oxidative stress response. Activates or represses expression of target genes, depending on the context. Plays a key role in cholesterol homeostasis by acting as a sensor of cholesterol excess: in low cholesterol conditions, translocates into the nucleus and represses expression of genes involved in defense against cholesterol excess, such as CD36. In excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal. Critical for redox balance in response to oxidative stress: acts by binding the AREs motifs on promoters and mediating activation of oxidative stress response genes, such as GCLC, GCLM, GSS, MT1 and MT2. Plays an essential role during fetal liver hematopoiesis: probably has a protective function against oxidative stress and is involved in lipid homeostasis in the liver. Involved in proteasome homeostasis: in response to proteasome inhibition, mediates the 'bounce-back' of proteasome subunits by translocating into the nucleus and activating expression of genes encoding proteasome subunits. Also involved in regulating glucose flux. Together with CEBPB.represses expression of DSPP during odontoblast differentiation. In response to ascorbic acid induction, activates expression of SP7/Osterix in osteoblasts. |
| Protein Name | Endoplasmic Reticulum Membrane Sensor Nfe2l1Locus Control Region-Factor 1Lcr-F1Nuclear Factor Erythroid 2-Related Factor 1Nf-E2-Related Factor 1Nfe2-Related Factor 1Nuclear Factor - Erythroid Derived 2 - Like 1Protein Nrf1 - P120 FormTranscription Factor 11Tcf-11 Cleaved Into - Transcription Factor Nrf1Protein Nrf1 - P110 Form |
| Cellular Localisation | Endoplasmic Reticulum Membrane Sensor Nfe2l1: Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinEndoplasmic Reticulum MembraneSingle-Pass Type Iii Membrane ProteinIn Normal ConditionsProbably Has A Single-Pass Type Ii Membrane Protein TopologyWith The Dna-Binding Domain Facing The Endoplasmic Reticulum LumenFollowing Cellular StressIt Is Rapidly And Efficiently Retrotranslocated To The Cytosolic Side Of The MembraneA Process Dependent On P97/VcpTo Have A Single-Pass Type Iii Membrane Protein Topology With The Major Part Of The Protein Facing The CytosolRetrotranslocated Proteins Are Normally Rapidly Degraded By The Proteasome And Active Species Do Not AccumulateHoweverRetrotranslocated Protein Nfe2l1 Escapes Degradation And Is Cleaved At Leu-104 By Ddi2Releasing The Protein From The Endoplasmic Reticulum Membrane And Forming The Transcription Factor Nrf1 That Translocates Into The NucleusTranscription Factor Nrf1: NucleusTranslocates Into The Nucleus Following Cleavage Of Endoplasmic Reticulum Membrane Sensor Nfe2l1 By Aspartyl Protease Ddi2 |
| Alternative Antibody Names | Anti-Endoplasmic Reticulum Membrane Sensor Nfe2l1 antibodyAnti-Locus Control Region-Factor 1 antibodyAnti-Lcr-F1 antibodyAnti-Nuclear Factor Erythroid 2-Related Factor 1 antibodyAnti-Nf-E2-Related Factor 1 antibodyAnti-Nfe2-Related Factor 1 antibodyAnti-Nuclear Factor - Erythroid Derived 2 - Like 1 antibodyAnti-Protein Nrf1 - P120 Form antibodyAnti-Transcription Factor 11 antibodyAnti-Tcf-11 Cleaved Into - Transcription Factor Nrf1 antibodyAnti-Protein Nrf1 - P110 Form antibodyAnti-NFE2L1 antibodyAnti-HBZ17 antibodyAnti-NRF1 antibodyAnti-TCF11 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
