| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NFATC3 (1-290) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFATC3 |
| Gene ID: |
4775 |
| Uniprot ID: |
NFAC3_HUMAN |
| Immunogen Region: |
1-290 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1). |
| Immunogen Sequence: |
MTTANCGAHDELDFKLVFGE DGAPAPPPPGSRPADLEPDD CASIYIFNVDPPPSTLTTPL CLPHHGLPSHSSVLSPSFQL QSHKNYEGTCEIPESKYSPL GGPKPFECPSIQITSISPNC HQELDAHEDDLQINDPEREF LERPSRDHLYLPLEPSYRES SLSPSPASSISSRSWFSDAS SCESLSHIYDDVDSELNEAA ARFTLGSPLTSPGGSPGGCP GEETWHQQYGLGHSLSPRQ |
| Tissue Specificity | Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle. |
| Post Translational Modifications | Phosphorylated by NFATC-kinase.dephosphorylated by calcineurin. |
| Function | Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Along with NFATC4, involved in embryonic heart development. |
| Protein Name | Nuclear Factor Of Activated T-Cells - Cytoplasmic 3Nf-Atc3Nfatc3NfatxT-Cell Transcription Factor Nfat4Nf-At4Nf-At4c |
| Database Links | Reactome: R-HSA-2025928Reactome: R-HSA-2871809Reactome: R-HSA-5607763 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated DephosphorylationRapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium SignalsThe Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 3 antibodyAnti-Nf-Atc3 antibodyAnti-Nfatc3 antibodyAnti-Nfatx antibodyAnti-T-Cell Transcription Factor Nfat4 antibodyAnti-Nf-At4 antibodyAnti-Nf-At4c antibodyAnti-NFATC3 antibodyAnti-NFAT4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and MSH6 knockout (KO) HeLa cells, using [KO Validated] MSH6 Rabbit polyclonal antibody (STJ119980) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98765/382817/STJ119980_1__09948.1713151312.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MSH6 knockout (KO) HeLa cells, using [KO Validated] MSH6 Rabbit polyclonal antibody (STJ119980) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
