• Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit polyclonal antibody (STJ24751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NFAT2 in paraffin-embedded human placenta using NFAT2 Rabbit polyclonal antibody (STJ24751) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse thymus, using NFAT2 Rabbit polyclonal antibody (STJ24751) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NFATC1 antibody (20-300) (STJ24751)

SKU:
STJ24751

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFAT2 (20-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFATC1
Gene ID: 4772
Uniprot ID: NFAC1_HUMAN
Immunogen Region: 20-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of NFAT2 (NP_765978.1).
Immunogen Sequence: VFGRGETLGPAPRAGGTMKS AEEEHYGYASSNVSPALPLP TAHSTLPAPCHNLQTSTPGI IPPADHPSGYGAALDGGPAG YFLSSGHTRPDGAPALESPR IEITSCLGLYHNNNQFFHDV EVEDVLPSSKRSPSTATLSL PSLEAYRDPSCLSPASSLSS RSCNSEASSYESNYSYPYAS PQTSPWQSPCVSPKTTDPEE GFPRGLGACTLLGSPRHSPS TSPRASVTEESWLGARSSR
Tissue Specificity Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.
Post Translational Modifications Phosphorylated by NFATC-kinase and GSK3B.phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1.
Function Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 1
Nf-Atc1
Nfatc1
Nfat Transcription Complex Cytosolic Component
Nf-Atc
Nfatc
Database Links Reactome: R-HSA-2025928
Reactome: R-HSA-2871809
Reactome: R-HSA-4086398
Reactome: R-HSA-5607763
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated Dephosphorylation
Rapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium Signals
The Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription
Nuclear Translocation Of Nfatc1 Is Enhanced In The Presence Of Tnfsf11
Nuclear Translocation Is Decreased In The Presence Of Fbn1 Which Can Bind And Sequester Tnfsf11
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 1 antibody
Anti-Nf-Atc1 antibody
Anti-Nfatc1 antibody
Anti-Nfat Transcription Complex Cytosolic Component antibody
Anti-Nf-Atc antibody
Anti-Nfatc antibody
Anti-NFATC1 antibody
Anti-NFAT2 antibody
Anti-NFATC antibody

Information sourced from Uniprot.org

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