• Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
  • Western blot analysis of 823 293T-UV HELA Cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-NFAT5 antibody (212-284 aa) (STJ98961)

SKU:
STJ98961

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nuclear factor of activated T-cells 5 (212-284 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:500-200
ELISA 1:10000-20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFAT5
Gene ID: 10725
Uniprot ID: NFAT5_HUMAN
Immunogen Region: 212-284 aa
Specificity: The antibody detects endogenous NFAT5
Immunogen: Synthetic peptide from the human protein at the amino acid range 212-284
Post Translational Modifications Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress.this phosphorylation mediates its rapid nuclear localization. Poly-ADP-ribosylated by PARP1 in response to DNA damage, promoting recruitment to sites of R-loop-associated DNA damage.
Function Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-ACTAGTGGAAACATATAATCCAATGGTGACCGCT-3'. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes.optimal transactivation of these genes requires the presence of DDX5/DDX17. Also involved in the DNA damage response by preventing formation of R-loops.R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.
Protein Name Nuclear Factor Of Activated T-Cells 5
Nf-At5
T-Cell Transcription Factor Nfat5
Tonicity-Responsive Enhancer-Binding Protein
Tone-Binding Protein
Tonebp
Cellular Localisation Nucleus
Cytoplasm
Chromosome
Nuclear Distribution Increases Under Hypertonic Conditions
Recruited To Sites Of R-Loop-Associated Dna Damage Following Poly-Adp-Ribosylation By Parp1
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells 5 antibody
Anti-Nf-At5 antibody
Anti-T-Cell Transcription Factor Nfat5 antibody
Anti-Tonicity-Responsive Enhancer-Binding Protein antibody
Anti-Tone-Binding Protein antibody
Anti-Tonebp antibody
Anti-NFAT5 antibody
Anti-KIAA0827 antibody
Anti-TONEBP antibody

Information sourced from Uniprot.org

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