• Immunohistochemistry analysis of paraffin-embedded human colon using NEUROG3 Rabbit polyclonal antibody (STJ24747) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using NEUROG3 antibody (STJ24747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NEUROG3 antibody (1-214) (STJ24747)

SKU:
STJ24747

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEUROG3 (1-214) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEUROG3
Gene ID: 50674
Uniprot ID: NGN3_HUMAN
Immunogen Region: 1-214
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-214 of human NEUROG3 (NP_066279.2).
Immunogen Sequence: MTPQPSGAPTVQVTRETERS FPRASEDEVTCPTSAPPSPT RTRGNCAEAEEGGCRGAPRK LRARRGGRSRPKSELALSKQ RRSRRKKANDRERNRMHNLN
Function Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types.
Protein Name Neurogenin-3
Ngn-3
Class A Basic Helix-Loop-Helix Protein 7
Bhlha7
Protein Atonal Homolog 5
Database Links Reactome: R-HSA-210744
Reactome: R-HSA-210746
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Neurogenin-3 antibody
Anti-Ngn-3 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 7 antibody
Anti-Bhlha7 antibody
Anti-Protein Atonal Homolog 5 antibody
Anti-NEUROG3 antibody
Anti-ATOH5 antibody
Anti-BHLHA7 antibody
Anti-NGN3 antibody

Information sourced from Uniprot.org

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