Anti-Neurofilament medium polypeptide antibody (549-845) (STJA0040900)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenThis antibody has been made against a recombinant fusion protein containing the extreme C-terminus of rat NF-M (amino acids 549-845) expressed in and purified from E. coli.
General Information
| Short Description | Rabbit polyclonal anti-Neurofilament medium polypeptide (549-845) for use in IF, ICC, IHC and WB in Bovine, Chicken, Horse, Human, Mouse, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IF/ICC/IHC/WB |
| Host | Rabbit |
| Reactivity | Bovine/Chicken/Horse/Human/Mouse/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:1000-1:5000IHC 1:1000-1:2500ICC 1:1000-1:2500 |
| Formulation | Lyophilized with sodium azide. |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
Target Information
| Gene Symbol | NEFM |
| Gene ID | 4741 |
| Uniprot ID | NFM_HUMAN |
| Immunogen | This antibody has been made against a recombinant fusion protein containing the extreme C-terminus of rat NF-M (amino acids 549-845) expressed in and purified from E. coli. |
| Immunogen Region | 549-845 |
| Immunogen Sequence | Rat |
| Specificity | Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. |
Additional Info
| Post Translational Modifications | There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Medium PolypeptideNf-M160 Kda Neurofilament ProteinNeurofilament 3Neurofilament Triplet M Protein |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionAxon |
| Alternative Antibody Names | Anti-Neurofilament Medium Polypeptide antibodyAnti-Nf-M antibodyAnti-160 Kda Neurofilament Protein antibodyAnti-Neurofilament 3 antibodyAnti-Neurofilament Triplet M Protein antibodyAnti-NEFM antibodyAnti-NEF3 antibodyAnti-NFM antibody |
Information sourced from Uniprot.org