Anti-Neurofilament light polypeptide antibody [R04-7H3] (STJA0033967)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of mouse 68kDa Neurofilament
STJA0033967
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General Information

Short DescriptionRabbit monoclonal anti-Neurofilament light polypeptide for use in WB, IHC-P and IP in Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IP
HostRabbit
ReactivityMouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR04-7H3
IsotypeIgG
ConjugationUnconjugated
Concentration0.3 mg/mL
PurificationAffinity Purified
Dilution RangeWB 1:500-1:1000
IHC 1:50-1:100
IP 1:20
Formulation50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolNefl
Gene ID18039
Uniprot IDNFL_MOUSE
ImmunogenRecombinant protein of mouse 68kDa Neurofilament

Additional Info

Tissue Specificity Expressed in the sciatic nerve (at protein level).
Post Translational Modifications O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Light Polypeptide
Nf-L
68 Kda Neurofilament Protein
Neurofilament Triplet L Protein
Database Links Reactome: R-MMU-438066
Reactome: -MMU-5673001
Cellular Localisation Cell Projection
Axon
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Neurofilament Light Polypeptide antibody
Anti-Nf-L antibody
Anti-68 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet L Protein antibody
Anti-Nefl antibody
Anti-Nf68 antibody
Anti-Nfl antibody

Information sourced from Uniprot.org

Citations

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