• Western blot analysis of extracts of various cell lines, using Neurofilament L antibody (STJ11103250) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of SH-SY5Y cells, using Neurofilament L antibody (STJ11103250) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded mouse brain using Neurofilament L rabbit monoclonal antibody (STJ11103250) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Neurofilament L rabbit monoclonal antibody (STJ11103250) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat brain using Neurofilament L rabbit monoclonal antibody (STJ11103250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using Neurofilament L rabbit monoclonal antibody (STJ11103250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of SH-SY5Y cells using 3ug Neurofilament L antibody (STJ11103250). Western blot was performed from the immunoprecipitate using Neurofilament L antibody (STJ11103250) at a dilition of 1:10000.

Anti-Neurofilament L antibody (STJ11103250)

SKU:
STJ11103250
Current Stock:
Host: Rabbit
Applications: WB, IHC, IF, IP
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit monoclonal antibody anti-Neurofilament L is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Clonality: Monoclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: NEFL
Gene ID: 4747
Uniprot ID: NFL_HUMAN
Immunogen: Recombinant protein of human Neurofilament L.
Post Translational Modifications O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Light Polypeptide
Nf-L
68 Kda Neurofilament Protein
Neurofilament Triplet L Protein
Database Links Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Projection
Axon
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Neurofilament Light Polypeptide antibody
Anti-Nf-L antibody
Anti-68 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet L Protein antibody
Anti-NEFL antibody
Anti-NF68 antibody
Anti-NFL antibody

Information sourced from Uniprot.org

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