• Western blot analysis of extracts of various cell lines, using NeuN antibody (STJ25310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of U2OS cells, using NeuN rabbit polyclonal antibody (STJ25310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using NeuN rabbit polyclonal antibody (STJ25310) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat brain cells using NeuN rabbit polyclonal antibody (STJ25310) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain cells using NeuN rabbit polyclonal antibody (STJ25310) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RBFOX3 antibody (1-220) (STJ25310)

SKU:
STJ25310

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NeuN (1-220) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBFOX3
Gene ID: 146713
Uniprot ID: RFOX3_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of NeuN (NP_001076044.1).
Immunogen Sequence: MAQPYPPAQYPPPPQNGIPA EYAPPPPHPTQDYSGQTPVP TEHGMTLYTPAQTHPEQPGS EASTQPIAGTQTVPQTDEAA QTDSQPLHPSDPTEKQQPKR LHVSNIPFRFRDPDLRQMFG QFGKILDVEIIFNERGSKGF GFVTFETSSDADRAREKLNG TIVEGRKIEVNNATARVMTN KKTGNPYTNGWKLNPVVGAV YGPEFYAVTGFPYPTTGTAV
Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Protein Name Rna Binding Protein Fox-1 Homolog 3
Fox-1 Homolog C
Neuronal Nuclei Antigen
Neun Antigen
Database Links Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Cytoplasm
Largely Restricted To Neuronal Nuclei
However
Significant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 3 antibody
Anti-Fox-1 Homolog C antibody
Anti-Neuronal Nuclei Antigen antibody
Anti-Neun Antigen antibody
Anti-RBFOX3 antibody

Information sourced from Uniprot.org

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