Western blot analysis of various lysates using NeuN Mouse mAb (STJ11107255) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-NeuN antibody [SMM5346] (STJ11107255)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG
STJ11107255
๐Ÿšš Free UK Delivery on orders over ยฃ150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionMouse monoclonal NeuN antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDSMM5346
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:2500-1:5000
IHC-P:1:200-1:800
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRBFOX3
Gene ID146713
Uniprot IDRFOX3_HUMAN
SpecificityRecombinant protein of human NeuN.

Additional Info

Function Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD).
Protein Name Rna Binding Protein Fox-1 Homolog 3
Fox-1 Homolog C
Neuronal Nuclei Antigen
Neun Antigen
Database Links Reactome: R-HSA-9768919
Cellular Localisation Nucleus
Cytoplasm
Largely Restricted To Neuronal Nuclei
However
Significant Cytoplasmic Localization In Neurons From Brains From Hiv-Infected Individuals With Cognitive Impairment
Alternative Antibody Names Anti-Rna Binding Protein Fox-1 Homolog 3 antibody
Anti-Fox-1 Homolog C antibody
Anti-Neuronal Nuclei Antigen antibody
Anti-Neun Antigen antibody
Anti-RBFOX3 antibody

Information sourced from Uniprot.org

Citations

Product Review

!