Anti-Nestin antibody (region 317-630 aa) [4D11] (STJA0040750)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1, kappa
ImmunogenPartial segment (region 317-630 aa) of human Nestin expressed in E.coli
General Information
| Short Description | Mouse monoclonal anti-Nestin (region 317-630 aa) for use in ICC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4D11 |
| Isotype | IgG1, kappa |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 1:1000-1:5000ICC 1:250-1:500 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
Target Information
| Gene Symbol | NES |
| Gene ID | 10763 |
| Uniprot ID | NEST_HUMAN |
| Immunogen | Partial segment (region 317-630 aa) of human Nestin expressed in E.coli |
| Immunogen Region | region 317-630 aa |
| Immunogen Sequence | Human |
| Specificity | This antibody is specific for the 240 kDa Nestin protein by WB on developing rat brain (P18) homogenate. A much weaker band at approx. 90 kDa may also be seen. This is suggested to be a breakdown product of the 240 kDa band. Human, Rodent. |
Additional Info
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org