Anti-Nestin antibody (315-630) (STJA0040988)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPart of recombinant human protein (amino acids 315-630).
General Information
| Short Description | Rabbit polyclonal anti-Nestin (315-630) for use in ICC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:1000-1:5000ICC 3.5138888888888888 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 ยตL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8ยฐC. After reconstitution divide into aliquots and store at-20ยฐC for long-term |
Target Information
| Gene Symbol | NES |
| Gene ID | 10763 |
| Uniprot ID | NEST_HUMAN |
| Immunogen | Part of recombinant human protein (amino acids 315-630). |
| Immunogen Region | 315-630 |
| Immunogen Sequence | Human |
| Specificity | Reacts with Human. Other species not tested. |
Additional Info
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org