Anti-NES antibody [4D11] (STJA0003696)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant human Nestin expressed in and purifeid from E. coli.
General Information
| Short Description | Mouse monoclonal anti-Nestin for use in WB, IHC and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 4D11 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | This antibody was protein g purified culture from supernatant. |
| Dilution Range | WB 1:2000IHC 1:500ICC 1:500 |
| Formulation | 100 ul in PBS + 50% Glycerol and 5 mm Sodium Azide |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NES |
| Gene ID | 10763 |
| Uniprot ID | NEST_HUMAN |
| Immunogen | Recombinant human Nestin expressed in and purifeid from E. coli. |
Additional Info
| Tissue Specificity | CNS stem cells. |
| Post Translational Modifications | Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Protein Name | Nestin |
| Database Links | |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Nestin antibodyAnti-NES antibodyAnti-Nbla00170 antibody |
Information sourced from Uniprot.org