Anti-Nephrin antibody [SM0481] (STJA0010481)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenKLH conjugated Synthetic peptide corresponding to Mouse Nephrin
General Information
| Short Description | Mouse monoclonal anti-Nephrin for use in IHC and IF in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF |
| Host | Mouse |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SM0481 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | IHC/IF (M) 1:500-1:1500 |
| Formulation | PBS with 0.15% ProClin300, 100 Mu g/mL BSA and 50% glycerol. |
| Storage Instruction | Store at-20C for up to one year, and avoid repeated freeze-thaw cycles. |
Target Information
| Gene Symbol | Nphs1 |
| Gene ID | 54631 |
| Uniprot ID | NPHN_MOUSE |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Nephrin |
Additional Info
| Post Translational Modifications | Phosphorylated at Tyr-1208 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-MMU-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocated At Podocyte Slit Diaphragm Between Podocyte Foot Processes |
| Alternative Antibody Names | Anti-Nephrin antibodyAnti-Renal Glomerulus-Specific Cell Adhesion Receptor antibodyAnti-Nphs1 antibodyAnti-Nphn antibody |
Information sourced from Uniprot.org