Anti-Nephrin antibody (1019-1241 aa) [SRM5386] (STJ11107297)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Nephrin (1019-1241 aa) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM5386 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NPHS1 |
| Gene ID | 4868 |
| Uniprot ID | NPHN_HUMAN |
| Immunogen Region | 1019-1241 aa |
| Immunogen Sequence | LGDSGLADKGTQLPITTPGL HQPSGEPEDQLPTEPPSGPS GLPLLPVLFALGGLLLLSNA SCVGGVLWQRRLRRLAEGIS EKTEAGSEEDRVRNEYEESQ WTGERDTQSSTVSTTEAEPY YRSLRDFSPQLPPTQEEVSY SRGFTGEDEDMAFPGHLYDE VERTYPPSGAWGPLYDEVQM GPWDLHWPEDTYQDPRGIYD QVAGDLDTLEPDSLPFELRG HLV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1019-1241 of human Nephrin (NP_004637.1). |
Additional Info
| Tissue Specificity | Specifically expressed in podocytes of kidney glomeruli. |
| Post Translational Modifications | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. Tyrosine phosphorylation is reduced by high glucose levels. Dephosphorylated by tensin TNS2 which leads to reduced binding of NPHN1 to PIK3R1. |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. |
| Protein Name | NephrinRenal Glomerulus-Specific Cell Adhesion Receptor |
| Database Links | Reactome: R-HSA-373753 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinPredominantly Located At Podocyte Slit Diaphragm Between Podocyte Foot ProcessesAlso Associated With Podocyte Apical Plasma Membrane |
| Alternative Antibody Names | Anti-Nephrin antibodyAnti-Renal Glomerulus-Specific Cell Adhesion Receptor antibodyAnti-NPHS1 antibodyAnti-NPHN antibody |
Information sourced from Uniprot.org