Anti-NELFE antibody (1-280) (STJ29086)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NELFE (1-280) antibody for use in WB, IF, ICC and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NELFE |
| Gene ID | 7936 |
| Uniprot ID | NELFE_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MLVIPPGLSEEEEALQKKFN KLKKKKKALLALKKQSSSST TSQGGVKRSLSEQPVMDTAT ATEQAKQLVKSGAISAIKAE TKNSGFKRSRTLEGKLKDPE KGPVPTFQPFQRSISADDDL QESSRRPQRKSLYESFVSSS DRLRELGPDGEEAEGPGAGD GPPRSFDWGYEERSGAHSSA SPPRSRSRDRSHERNRDRDR DRERDRDRDRDRDRERDRDR DRDRDRDRERDRDRERDRD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NELFE (NP_002895.3). |
Additional Info
| Tissue Specificity | Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. |
| Post Translational Modifications | Phosphorylated by the P-TEFb complex at sites next to its RNA recognition motif, promoting its release from chromatin. Sumoylated. Poly-ADP-ribosylated by PARP1, thereby preventing RNA-binding and relieving transcription pausing. |
| Function | Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. |
| Protein Name | Negative Elongation Factor ENelf-ERna-Binding Protein Rd |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeLocalizes To ChromatinPhosphorylation By The P-Tefb Complex Promotes Its Release From Chromatin |
| Alternative Antibody Names | Anti-Negative Elongation Factor E antibodyAnti-Nelf-E antibodyAnti-Rna-Binding Protein Rd antibodyAnti-NELFE antibodyAnti-RD antibodyAnti-RDBP antibody |
Information sourced from Uniprot.org