Anti-NEK7 antibody (274-286) (STJ72426)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-NEK7 (274-286) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30-33kDa band observed in Human Lung and Testis lysates and in Mouse and Rat Lung and Testis lysates (calculated MW of 34.6kDa according to Human NP_598001.1 and 34.5kDa accordi |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | NEK7 |
| Gene ID | 140609 |
| Uniprot ID | NEK7_HUMAN |
| Accession Number | NP_598001.1 |
| Immunogen Region | 274-286 |
| Immunogen Sequence | CINPDPEKRPDVT |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-195 required for its activation. |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. Phosphorylates RPS6KB1. Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity. Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis. |
| Protein Name | Serine/Threonine-Protein Kinase Nek7Never In Mitosis A-Related Kinase 7Nima-Related Protein Kinase 7 |
| Database Links | Reactome: R-HSA-2980767Reactome: R-HSA-3301854Reactome: R-HSA-9648025 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle PoleMicrotubule Organizing CenterCentrosomePresent At Centrosome Throughout The Cell CycleAlso Detected At Spindle Midzone Of The Anaphase Cells And Eventually Concentrates At The MidbodyInteraction With Anks3 Prevents Its Translocation To The Nucleus |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Nek7 antibodyAnti-Never In Mitosis A-Related Kinase 7 antibodyAnti-Nima-Related Protein Kinase 7 antibodyAnti-NEK7 antibody |
Information sourced from Uniprot.org