Anti-NEFH antibody (873-1020) (STJ110740)

Name: Anti-NEFH antibody (873-1020) (STJ110740)
SKU: STJ110740
Availability: InStock

⇓ Datasheet ⇓ SDS Citations

SPECIFICATIONS

ClonalityPolyclonal

HostRabbit

ConjugationUnconjugated

IsotypeIgG

STJ110740

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Short Description	Rabbit polyclonal NEFH (873-1020) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications	WB/IF/ICC/IP/ELISA
Host	Rabbit
Reactivity	Human/Mouse/Rat
Note	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Clonality	Polyclonal
Isotype	IgG
Conjugation	Unconjugated
Concentration	Lot specific
Purification	Affinity purification
Dilution Range	WB:1:500-1:1000 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Formulation	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Storage Instruction	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol	NEFH
Gene ID	4744
Uniprot ID	NFH_HUMAN
Immunogen Region	873-1020
Immunogen Sequence	KVEEKKEPAVEKPKESKVEA KKEEAEDKKKVPTPEKEAPA KVEVKEDAKPKEKTEVAKKE PDDAKAKEPSKPAEKKEAAP EKKDTKEEKAKKPEEKPKTE AKAKEDDKTLSKEPSKPKAE KAEKSSSTDQKDSKPPEKAT EDKAAKGK
Specificity	Recombinant fusion protein containing a sequence corresponding to amino acids 873-1020 of human Neurofilament H (NP_066554.2).

Post Translational Modifications	There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function	Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name	Neurofilament Heavy Polypeptide Nf-H 200 Kda Neurofilament Protein Neurofilament Triplet H Protein
Database Links
Cellular Localisation	Cytoplasm Cytoskeleton Cell Projection Axon
Alternative Antibody Names	Anti-Neurofilament Heavy Polypeptide antibody Anti-Nf-H antibody Anti-200 Kda Neurofilament Protein antibody Anti-Neurofilament Triplet H Protein antibody Anti-NEFH antibody Anti-KIAA0845 antibody Anti-NFH antibody

Information sourced from Uniprot.org