Anti-NEDD9 antibody (332-382 aa) (STJ192909)

SKU:
STJ192909

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Enhancer of filamentation 1 (332-382 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEDD9
Gene ID: 4739
Uniprot ID: CASL_HUMAN
Immunogen Region: 332-382 aa
Specificity: CASL Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from part of the human protein at the amino acid range 332-382
Post Translational Modifications Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected. Polyubiquitinated by ITCH/AIP4, leading to proteasomal degradation. PTK2/FAK1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins) following integrin stimulation. The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. Phosphorylation is required to recruit NEDD9 to T-cell receptor microclusters at the periphery of newly formed immunological synapses. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRKL and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross-linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium- and protein kinase C-dependent mechanisms and requires the integrity of the actin cytoskeleton. Phosphorylation at Ser-369 induces proteasomal degradation. Phosphorylated by LYN. Phosphorylation at Ser-780 by CSNK1D or CSNK1E, or phosphorylation of Thr-804 by CSNK1E enhances the interaction of NEDD9 with PLK1.
Function Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. As a focal adhesion protein, plays a role in embryonic fibroblast migration. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form. Promotes adhesion and migration of lymphocytes.as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs. Plays a role in the organization of T-cell F-actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse. Negatively regulates cilia outgrowth in polarized cysts. Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin. Positively regulates RANKL-induced osteoclastogenesis. Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory.
Protein Name Enhancer Of Filamentation 1
Hef1
Crk-Associated Substrate-Related Protein
Cas-L
Casl
Cas Scaffolding Protein Family Member 2
Cass2
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9
Nedd-9
Renal Carcinoma Antigen Ny-Ren-12
P105 Cleaved Into - Enhancer Of Filamentation 1 P55
Cellular Localisation Cytoplasm
Cell Cortex
Nucleus
Golgi Apparatus
Cell Projection
Lamellipodium
Cell Junction
Focal Adhesion
Cytoskeleton
Spindle Pole
Cilium
Cilium Basal Body
Basolateral Cell Membrane
Enhancer Of Filamentation 1 P55: Cytoplasm
Spindle
Alternative Antibody Names Anti-Enhancer Of Filamentation 1 antibody
Anti-Hef1 antibody
Anti-Crk-Associated Substrate-Related Protein antibody
Anti-Cas-L antibody
Anti-Casl antibody
Anti-Cas Scaffolding Protein Family Member 2 antibody
Anti-Cass2 antibody
Anti-Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9 antibody
Anti-Nedd-9 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-12 antibody
Anti-P105 Cleaved Into - Enhancer Of Filamentation 1 P55 antibody
Anti-NEDD9 antibody
Anti-CASL antibody

Information sourced from Uniprot.org

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