• Western blot analysis of various lysates using NECAP2 Rabbit polyclonal antibody (STJ116558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NECAP2 antibody (194-263) (STJ116558)

SKU:
STJ116558

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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NECAP2 (194-263) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NECAP2
Gene ID: 55707
Uniprot ID: NECP2_HUMAN
Immunogen Region: 194-263
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 194-263 of human NECAP2 (NP_060560.1).
Immunogen Sequence: GKTSTLIPPPGEQLAVGGSL VQPAVAPSSGGAPVPWPQPN PATADIWGDFTKSTGSTSSQ TQPGTGWVQF
Function Involved in endocytosis.
Protein Name Adaptin Ear-Binding Coat-Associated Protein 2
Necap Endocytosis-Associated Protein 2
Necap-2
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasmic Vesicle
Clathrin-Coated Vesicle Membrane
Cell Membrane
Colocalizes With Ap-2 At The Plasma Membrane
Alternative Antibody Names Anti-Adaptin Ear-Binding Coat-Associated Protein 2 antibody
Anti-Necap Endocytosis-Associated Protein 2 antibody
Anti-Necap-2 antibody
Anti-NECAP2 antibody

Information sourced from Uniprot.org

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