• Western blot analysis of extracts of various cell lines, using NDUFV1 rabbit polyclonal antibody antibody (STJ110320) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of various cell lines, using NDUFV1 rabbit polyclonal antibody antibody (STJ110320) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using NDUFV1 rabbit polyclonal antibody (STJ110320) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver using NDUFV1 rabbit polyclonal antibody (STJ110320) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using NDUFV1 rabbit polyclonal antibody (STJ110320) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NDUFV1 antibody (72-420) (STJ110320)

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STJ110320

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFV1 (72-420) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFV1
Gene ID: 4723
Uniprot ID: NDUV1_HUMAN
Immunogen Region: 72-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 72-420 of human NDUFV1 (NP_009034.2).
Immunogen Sequence: GPDWILGEIKTSGLRGRGGA GFPTGLKWSFMNKPSDGRPK YLVVNADEGEPGTCKDREIL RHDPHKLLEGCLVGGRAMGA RAAYIYIRGEFYNEASNLQV AIREAYEAGLIGKNACGSGY DFDVFVVRGAGAYICGEETA LIESIEGKQGKPRLKPPFPA DVGVFGCPTTVANVETVAVS PTICRRGGTWFAGFGRERNS GTKLFNISGHVNHPCTVEEE MSVPLKELIEKHAGGVTGG
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor.
Protein Name Nadh Dehydrogenase Ubiquinone Flavoprotein 1 - Mitochondrial
Complex I-51kd
Ci-51kd
Nadh Dehydrogenase Flavoprotein 1
Nadh-Ubiquinone Oxidoreductase 51 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Flavoprotein 1 - Mitochondrial antibody
Anti-Complex I-51kd antibody
Anti-Ci-51kd antibody
Anti-Nadh Dehydrogenase Flavoprotein 1 antibody
Anti-Nadh-Ubiquinone Oxidoreductase 51 Kda Subunit antibody
Anti-NDUFV1 antibody
Anti-UQOR1 antibody

Information sourced from Uniprot.org

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