| Short Description : | Rabbit polyclonal anti-NADH dehydrogenase iron-sulfur protein 5 (57-106) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC/IF/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 1 mg/mL |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NDUFS5 |
| Gene ID: | 4725 |
| Uniprot ID: | NDUS5_HUMAN |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human NDUFS5. AA range:57-106 |
| Immunogen Region: | 57-106 |
| Specificity: | NDUFS5 Polyclonal Antibody detects endogenous levels of NDUFS5 protein. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5Complex I-15 KdaCi-15 KdaNadh-Ubiquinone Oxidoreductase 15 Kda Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMitochondrion Intermembrane Space |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 5 antibodyAnti-Complex I-15 Kda antibodyAnti-Ci-15 Kda antibodyAnti-Nadh-Ubiquinone Oxidoreductase 15 Kda Subunit antibodyAnti-NDUFS5 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ATPIF1 knockout (KO) 293T cells, using [KO Validated] ATPIF1 Rabbit polyclonal antibody (STJ11100822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89758/358234/STJ11100822_1__18532.1713122566.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATPIF1 knockout (KO) 293T cells, using [KO Validated] ATPIF1 Rabbit polyclonal antibody (STJ11100822) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
