• Western blot analysis of extracts of various cell lines, using NDUFS4 rabbit monoclonal antibody (STJ11102353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of NIH-3T3 cells using NDUFS4 Rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of HepG2 cells, using NDUFS4 rabbit monoclonal antibody (STJ11102353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of C6 cells using NDUFS4 Rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat ovary using NDUFS4 rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of NDUFS4 in paraffin-embedded human colon carcinoma tissue using NDUFS4 Rabbit monoclonal antibody (STJ11102353) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human thyroid cancer using NDUFS4 rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of NDUFS4 in paraffin-embedded human liver tissue using NDUFS4 Rabbit monoclonal antibody (STJ11102353) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using NDUFS4 rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from HepG2 cells, using NDUFS4 Rabbit monoclonal antibody (STJ11102353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of C6 cells using NDUFS4 rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDUFS4 Rabbit monoclonal antibody (STJ11102353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of NIH-3T3 cells using NDUFS4 rabbit monoclonal antibody (STJ11102353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NDUFS4 antibody (1-100) [S3MR] (STJ11102353)

SKU:
STJ11102353

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NDUFS4 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS4
Gene ID: 4724
Uniprot ID: NDUS4_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NDUFS4 (O43181).
Immunogen Sequence: MAAVSMSVVLRQTLWRRRAV AVAALSVSRVPTRSLRTSTW RLAQDQTQDTQLITVDEKLD ITTLTGVPEEHIKTRKVRIF VPARNNMQSGVNNTKKWKME
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial
Complex I-18 Kda
Ci-18 Kda
Complex I-Aqdq
Ci-Aqdq
Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
The Interaction With Bcap31 Mediates Mitochondria Localization
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 4 - Mitochondrial antibody
Anti-Complex I-18 Kda antibody
Anti-Ci-18 Kda antibody
Anti-Complex I-Aqdq antibody
Anti-Ci-Aqdq antibody
Anti-Nadh-Ubiquinone Oxidoreductase 18 Kda Subunit antibody
Anti-NDUFS4 antibody

Information sourced from Uniprot.org

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