• Immunohistochemistry analysis of paraffin-embedded rat liver using NDUFS3 Rabbit monoclonal antibody (STJ11102115) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NDUFS3 Rabbit monoclonal antibody (STJ11102115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDUFS3 antibody (165-264) [S5MR] (STJ11102115)

SKU:
STJ11102115

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-NDUFS3 (165-264) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS3
Gene ID: 4722
Uniprot ID: NDUS3_HUMAN
Immunogen Region: 165-264
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 165-264 of human NDUFS3 (O75489).
Immunogen Sequence: WYEREIWDMFGVFFANHPDL RRILTDYGFEGHPFRKDFPL SGYVELRYDDEVKRVVAEPV ELAQEFRKFDLNSPWEAFPV YRQPPESLKLEAGDKKPDAK
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 3 - Mitochondrial
Complex I-30kd
Ci-30kd
Nadh-Ubiquinone Oxidoreductase 30 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Reactome: R-HSA-9013408
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 3 - Mitochondrial antibody
Anti-Complex I-30kd antibody
Anti-Ci-30kd antibody
Anti-Nadh-Ubiquinone Oxidoreductase 30 Kda Subunit antibody
Anti-NDUFS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance