• Western blot analysis of extracts of various cell lines, using NDUFS1 rabbit polyclonal antibody (STJ119236) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using NDUFS1 rabbit polyclonal antibody (STJ119236) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using NDUFS1 rabbit polyclonal antibody (STJ119236) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using NDUFS1 rabbit polyclonal antibody (STJ119236) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using NDUFS1 rabbit polyclonal antibody (STJ119236) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NDUFS1 antibody (80-290) (STJ119236)

SKU:
STJ119236

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS1 (80-290) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS1
Gene ID: 4719
Uniprot ID: NDUS1_HUMAN
Immunogen Region: 80-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 80-290 of human NDUFS1 (NP_004997.4).
Immunogen Sequence: VEIEKAPKVVAACAMPVMKG WNILTNSEKSKKAREGVMEF LLANHPLDCPICDQGGECDL QDQSMMFGNDRSRFLEGKRA VEDKNIGPLVKTIMTRCIQC TRCIRFASEIAGVDDLGTTG RGNDMQVGTYIEKMFMSELS GNIIDICPVGALTSKPYAFT ARPWETRKTESIDVMDAVGS NIVVSTRTGEVMRILPRMHE DINEEWISDKT
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for catalysing the entry and efficient transfer of electrons within complex I. Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes.
Protein Name Nadh-Ubiquinone Oxidoreductase 75 Kda Subunit - Mitochondrial
Complex I-75kd
Ci-75kd
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh-Ubiquinone Oxidoreductase 75 Kda Subunit - Mitochondrial antibody
Anti-Complex I-75kd antibody
Anti-Ci-75kd antibody
Anti-NDUFS1 antibody

Information sourced from Uniprot.org

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